• 文献标题:   Nitrogen-doped graphene quantum dot-based sensing platform for metabolite detection
  • 文献类型:   Article
  • 作  者:   LIU XT, SU XG
  • 作者关键词:   nitrogendoped graphene quantum dot, fluorescent sensing, metabolite, cysteine
  • 出版物名称:   MICROCHIMICA ACTA
  • ISSN:   0026-3672 EI 1436-5073
  • 通讯作者地址:   Jilin Univ
  • 被引频次:   0
  • DOI:   10.1007/s00604-020-04484-4
  • 出版年:   2020

▎ 摘  要

A novel fluorescent sensing platform based on nitrogen-doped graphene quantum dots (N-GQDs) is presented, which is able to detect various metabolites (cholesterol, glucose, lactate, and xanthine) rapidly, sensitively, and selectively. Hg(2+)can attach on the surface of N-GQDs, leading to the quenching of N-GQD fluorescence. In the presence of cysteine (Cys), Hg2+ is released from N-GQDs and associates with Cys. Then, the fluorescence of N-GQDs is recovered. Hydrogen peroxide, resulting from the enzymatic oxidation of metabolites, can convert two molecules of Cys into one molecule of cystine, which cannot bind with Hg2+. So, the fluorescence of N-GQDs quenched again. For cholesterol, glucose, lactate, and xanthine, the limits of detection are 0.035 mu mol/L, 0.025 mu mol/L, 0.07 mu mol/L, and 0.04 mu mol/L, respectively, and the linear ranges are 1-12 mu mol/L, 0.06-3 mu mol/L, 0.2-70 mu mol/L, and 0.12-17 mu mol/L, respectively. The presented method was applied to quantify metabolites in human blood samples with satisfactory results.