▎ 摘　　要

A metal ion ligands and molecular imprinted electrochemical sensors with double recognition patterns were prepared using molecular imprinting technique and with copper ion coordination unit as the double recognition mode. L-cystine (L-cys) was firstly assembled on the surface of graphene (rGO) and gold nanoparticles (AuNPs ) modified glassy carbon electrode by Au-S bond , and then the Cu-THR complex was assembled on the electrode surface by the coordination of L-cys and thrombin (Tn) by layer-by-layer self-assembly method. Thionine was used as monomer and electropolymerization method was used to form the imprinted membrane. The composite nanomaterials were characterized by scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) . The performance of the sensor was studied by cyclic voltammetry (CV) , electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) . Under the optimum conditions , the sensor showed an excellent linear relationship with thrombin concentration in the range of 2. 0 x 10(-9) - 5. 0 x 10(-7) g/L. The linear equation was -Delta I (mu A) = 17.73+1. 841gc (g/L) . The kinetic adsorption model of THR molecularly imprinted electrochemical sensor was investigated , and the measured imprinting factor p of the imprinted sensor was 4. 32 , with the binding rate constant k of 5. 68 s. The sensor had good stability and reproducibility , and could be used to detect thrombin in practice.