▎ 摘　　要

A novel sensitive electrochemical sensor has been developed by modification of glassy carbon electrode (GCE) with graphene (GRP), chitosan (CHIT), and bismuth oxide (Bi2O3) nanoparticles. The morphological characteristics of nanocomposite (GRP-CHIT-Bi2O3 or GCB) were studied by scanning electron microscope and atomic force microscopy. The electrochemical behavior of epinephrine at nanocomposite modified GCE (GCB/GCE) was investigated in pH 7.4 phosphate buffer solution using cyclic voltammetry and square wave voltammetry. GCB/GCE showed an enhancement in the current response as compared to bare GCE. Electrochemical impedance spectra showed a reduction of charge transfer resistance and higher electrocatalytic behavior of the sensor. The electrooxidation process of epinephrine at the modified sensor was found to be diffusion controlled. GCB/GCE showed a linear response to epinephrine in the range 100 to 500 nM. The limit of detection and limit of quantification were found to be 3.56 and 11.85 nM, respectively, which is lower than many other sensors reported for epinephrine in literature. The modified sensor showed high sensitivity (1.3 nA/nM) and selectivity for epinephrine. The method was employed for quantification of epinephrine in pharmaceutical formulation and human blood serum samples.