▎ 摘　　要

Graphene quantum dots/Ag nanoparticles nanocomposite (GQDs/ AgNPs) was prepared via in. situ growth of AgNPs on the surface of GQDs, in which GQDs served as both reducing agent and stabilizer. The as. prepared nanocomposites were mono-dispersed with an average diameter of less than 30 nm. The obtained GQDs@ AgNPs nanocomposites exhibited excellent intrinsic peroxidese-like activity, which could catalyze the oxidization of 3,3', 5,5'-tetramethylbenzidine (TMB) by H2O2 to produce a colour product. Steady. state kinetic assays showed that the catalytic activity of GQDs/ AgNPs towards H2O2 fitted well with typical Michaelis-Menten kinetic model, followed by the ping-pong mechanism. Compared with the horseradish peroxidase (HRP), GQDs/ AgNPs showed higher affinity towards TMB and H2O2 substrate. Based on the intrinsic peroxidese-like activity of GQDs/ AgNPs nanocomposites and the production of H2O2 after the oxidation of glucose, a colorimetric method was developed for the detection of H2O2 and glucose, along with detection limit of 0. 18 mu mol/L and 1. 6 mu mol/L, respectively. The present method was applied to the detection of glucose in human serum, and the obtained results agreed with that obtained by reference method.