▎ 摘　　要

In this study, a prototype technique was used to develop cylindrical bone graft substitute (C-BGS) from gelatin (GEL) derived from fish skin, demineralized bone matrix (DBM) from fishbone, reduced graphene oxide (R-GO), and nanocurcumin (Nano-Cu) for their potential application in bone graft substitute. The impact of the inclusion of R-GO and Nano-Cu on the physicochemical characteristics, antibacterial, and in vitro properties of the C-BGS was assessed. The C-BGS showed compressive strength of 13.64 +/- 0.20 (MPa) and elongation at break (%) of 23.76 +/- 0.20. The antibacterial activity of C-BGS against E.coli and S.aureus was excellent. In vitro study, revealed 98% cell viability on the C-BGS. The C-BGS with physicochemical, antibacterial, and biocompatibility properties can tried as a bone graft substitute material.