▎ 摘　　要

Uric acid (UA), as vital biological substance, is desirable to be accurate detected in clinical monitoring and diagnosis. Sensitive electrochemical biosensor for detection of UA was constructed with cationic poly(diallyldimethylammonium chloride) functionalized reduced graphene oxide (PDDA-rGO) and anionic Au nanoparticles mixed with polyoxometalates clusters K8P2W16V2O62 (P2W16V2-Au). The effective combination of P2W16V2-Au and PDDA-rGO brings many advantages in electrochemical detection, involving the fast transmission of electric charges, the unimpeded pathways for diffusion and more sensing sites. Under optimized conditions, the proposed sensor showed acceptable analytical performances for UA in terms of good linearity (over the concentration range from 2.5 x 10(-7) to 1.5 x 10(-4) M) and lower detection limit 8.0 x 10(-8) M (S/IN =3). Furthermore, the superior cycling stability, reproducibility, and selectivity make the electrochemical sensor suitable for detecting the UA in urine assay. (C) 2016 Elsevier B.V. All rights reserved.