• 文献标题:   Luminol, horseradish peroxidase, and glucose oxidase ternary functionalized graphene oxide for ultrasensitive glucose sensing
  • 文献类型:   Article
  • 作  者:   LI F, MA WJ, LIU JC, WU X, WANG Y, HE JB
  • 作者关键词:   graphene oxide, horseradish peroxidase, glucose oxidase, glucose, chemiluminescence
  • 出版物名称:   ANALYTICAL BIOANALYTICAL CHEMISTRY
  • ISSN:   1618-2642 EI 1618-2650
  • 通讯作者地址:   Hefei Univ Technol
  • 被引频次:   1
  • DOI:   10.1007/s00216-017-0752-5
  • 出版年:   2018

▎ 摘  要

Luminol, horseradish peroxidase (HRP), and glucose oxidase (GOx) ternary functionalized graphene oxide (HRP/GOx-luminol-GO) with excellent chemiluminescence (CL) activity and specific enzymatic property was prepared via a simple and general strategy for the first time. In this approach, luminol functionalized GO (luminol-GO) was prepared by gently stirring GO with luminol. Then HRP and GOx were further co-immobilized onto the surface of luminol-GO by storing HRP and GOx with luminol-GO at 4 A degrees C overnight, to form HRP/GOx-luminol-GO bionanocomposites. The synthesized HRP/GOx-luminol-GO could react with H2O2 generated from GOx catalyzed glucose oxidization reaction, to produce strong CL emission in the presence of co-immobilized HRP. Thus, we developed an ultrasensitive, homogeneous, reagentless, selective, and simple CL sensing system for glucose detection. The resulting biosensors exhibited ultra-wide linear range from 5.0 nM to 5.0 mM, and an ultra-low detection limit of 1.2 nM, which was more than 3 orders of magnitude lower than previously reported methods. Furthermore, the sensing system was successfully applied for the detection of glucose in human blood samples.