• 文献标题:   Graphene-Gold Nanoparticle-modified Electrochemical Sensor for Detection of Kanamycin Based on Target-induced Aptamer Displacement
  • 文献类型:   Article
  • 作  者:   ZHENG JY, FENG RR, HE CM, LI XX
  • 作者关键词:   kanamycin, electrochemical aptasensor, graphene, gold nanoparticle, methylene blue
  • 出版物名称:   CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
  • ISSN:   1005-9040 EI 2210-3171
  • 通讯作者地址:   Yanan Univ
  • 被引频次:   1
  • DOI:   10.1007/s40242-018-8185-z
  • 出版年:   2018

▎ 摘  要

A highly sensitive and selective label-free electrochemical sensor was developed for the determination of kanamycin. To improve the sensitivity of the electrochemical sensor, graphene-gold nanoparticles were prepared by a one-step electrochemical coreduction process and were modified on the surface of a glassy carbon electrode. The double-stranded DNA(ds-DNA) duplex probe was immobilized onto the graphene-gold nanoparticle-modified electrode. The introduction of target kanamycin induced the displacement of aptamer from the ds-DNA duplex into the solution. Methylene blue(MB) as a redox indicator monitored the current change using differential pulse voltammetry. Under optimal conditions, the designed electrochemical aptasensor exhibited a wide linear range from 0.1 pmol/L to 10 pmol/L with a detection limit of 0.03 pmol/L for kanamycin. The experimental strategy enabled the direct analysis of milk samples, and the results showed high sensitivity and good selectivity.