▎ 摘　　要

The purpose of this report was to progress the advancement of nanotechnology combined with near-infrared (NIR) laser-mediated treatment for enhancing efficiency against breast cancer (MCF-7) cells. The physic-chemical interactions and properties of the surface improved nano-Graphene oxide (nGO) sheets with Copper Sulfide (CuS) quantum dots were analyzed by selected exclusive analytical methods. The cytotoxicity effect of nGO was investigated by various in vitro assays. The surface activated nGO was exhibited its maximum absorption peak at 233 nm. The XRD analysis shows that the intensity of graphite was decreased and new peak arises around 2 circle minus =11.40 with an interlayer distance of 0.728 to 0.828 nm. In addition, microscopic studies demonstrated that the synthesized nGO was found as a transparent layer with the crispy structure as well as few layers appear as nanoflakes. The cell toxicity enhancement on MCF-7 breast cancer cells was highly influenced by the concentration of CuSQDs loaded nG0 sheets with an assessed 1050 value was 100 mu g/mL. The fluorescence microscopic visualization was confirmed the cells apoptotic morphological variations and cell death in CuSQDs-nGO treated breast cancer (MCF-7) cell line. Furthermore, the augmented level of Caspase-3, ROS and LDH activities of cancer cells were exhibited after CuSQDs-nGO treatment. Therefore, the present detailed biological investigations demonstrated that NIR-combined with surface activated CuSQDs-nGO presented a significant enhancement of cytotoxic effect against MCF-7 breast cancer cell lines by photothermal therapy (PTT).