▎ 摘　　要

Gold nanoparticles (AuNPs) modified with nitrogen-doped and nanoperforated graphene (DNPG) were successfully fabricated on a glassy carbon electrode (GCE). The composite showed excellent electrochemical response for hydrogen peroxide (H2O2) determination. Nitrogen-doped graphene has peroxidase-like activities. DNPG provided abundant surface area to support AuNPs, as well as ion transport shortcuts through the pores on DNPG sheets. DNPG and one-step electrode position of gold nanostructures can greatly improve conductivity and catalytic performance. The electroactive surface area of AuNPs/DNPG/GCE is almost 2.07 times higher than that of bare GCE. When AuNPs/DNPG/GCE were employed for non-enzymatic H2O2 sensing, the modified electrode presented an ultrahigh sensitivity of 5.3678 x 10(-4) mA mu mol L-1 cm(-2) in the range of 0.5-10 mmol L-1, as well as excellent selectivity, reproducibility and stability. AuNPs/DNPG/GCE could serve as an electrochemical biosensor for the detection of disease biomarkers in clinical practice.