• 文献标题:   DNA/Au-Pt bimetallic nanoparticles/graphene oxide-chitosan composites modified pencil graphite electrode used as an electrochemical biosensor for sub-picomolar detection of anti-HIV drug zidovudine
  • 文献类型:   Article
  • 作  者:   HASANJANI HRA, ZAREI K
  • 作者关键词:   deoxyribonucleic acid, zidovudine, graphen oxide, chitosan, aupt bimetallic nanoparticle
  • 出版物名称:   MICROCHEMICAL JOURNAL
  • ISSN:   0026-265X EI 1095-9149
  • 通讯作者地址:  
  • 被引频次:   11
  • DOI:   10.1016/j.microc.2021.106005 EA FEB 2021
  • 出版年:   2021

▎ 摘  要

Zidovudine (ZDV) has been widely employed in the human immunodeficiency virus type 1 (HIV-1) infection treatment. The short half-life, side effects of high doses, and adverse effects of ZDV necessitate the existence of a fast, simple, and reliable analysis method. In this work, a modified pencil graphite electrode was made using deoxyribonucleic acid/Au-Pt bimetallic nanoparticles/graphene oxide-chitosan (DNA/Au-Pt BNPs/GO-chit/PGE) for the determination of ZDV. Initially, the bare PGE was immersed in the GO-chit solution to create the graphene oxide-chitosan/pencil graphite electrode (GO-chit/PGE). Subsequently, electrodeposition of Au-Pt bimetallic nanoparticles (Au-Pt BNPs) was accomplished on the surface of GO-chit/PGE modified electrode by cyclic voltammetry (CV). Ultimately, DNA was immobilized on the Au-Pt BNPs/GO-chit/PGE applying a constant potential 0.5 V. The electrochemical response of ZDV upon the DNA/Au-Pt BNPs/GO-chit/PGE was measured by applying differential pulse voltammetry (DPV). In the optimum conditions, the proposed sensor displayed a wide linear dynamic range from 0.01 pM to 10.0 nM, a detection limit of 0.003 pM, and high selectivity and well reproducibility. Furthermore, the DNA/Au-Pt BNPs/GO-chit/PGE was successfully utilized to determine ZDV in human serum samples.