▎ 摘　　要

In the field of neural regeneration, a channel guidance conduit with the potential of self-electrical stimuli would be considered a suitable candidate. In this study, the core-shell conduit structure composed of PCL-chitosan-gelatin-Al2O3 (shell) and gellan-agar-polyaniline-graphene (core) was fabricated. For nanofiber fabrication, the co-electrospinning technique was utilized and in-situ chemical oxidative polymerization was the method of polyaniline-graphene synthesis. The morphology, chemical, and structural behavior of electrospun nanofibers were characterized using SEM, EDX, FTIR and XRD. Polyaniline-graphene nanocomposite properties were studied with FTIR and XRD. Also, output voltage, rheology, gelation time, and swelling demeanor were evaluated. The successful synthesis of PCL-chitosan-gelatin-Al2O3 nanofibers and polyaniline-graphene was detected using FTIR and XRD spectra. The piezoelectricity property of core-shell conduit demonstrates that the output voltage of the structure has been increased from 339 to 480 mV with 0 and 1.5 (wt.%) of polyaniline-graphene samples respectively. The changes in rheological parameters (complex viscosity, storage, and loss modulus) in the cases of polyaniline-graphene nanocomposite incorporation have been observed. The MTT assay of PC12 cells in contact with neural conduit did not show any specific toxicity. Obtained results depict that the thermosensitive gellan-agar- polyaniline-graphene-filled PCL-chitosan-gelatin-Al2O3 nanofibers would be a possible candidate as a conduit.