▎ 摘　　要

The porous graphene ( PG) with excellent structure was successfully prepared by a simple pyrolysis process, which was then applied to the construction of electrochemical sensor ( PG @ GCE ) for detection of calycosin. The porous graphene was characterized by Raman spectroscopy, Fourier transform infrared spectroscopy, and scanning electron microscopy. Also, the electrochemical properties of the proposed sensors were characterized by cyclic voltammetry ( CV ) and electrochemical impedance spectroscopy ( EIS), indicating that the sensor had relatively large specific surface area and higher electron transport rate. Based on all those characteristics , the PC @ GCE sensor displayed a great response to calycosin , a traditional Chinese medicine ( TCM ) active ingredient. Under the optimized conditions , the sensors displayed a good linear relationship between the peak current and the calycosin concentration in the range of 1. 8 x 10(-7) - 4. 4 x 10(-5) mol/L with a detection limit of 5. 8 x 10(-8) mol/L ( S/N = 3 ) . This study provided a novel analytical method for trace detection of calycosin, and rapid identification of Radix Hedysari and Radix Astragali in biological samples. At the same time, it would also deepen the application of porous graphene in the field of pharmaceutical analysis.