▎ 摘　　要

An easy one-pot polylol approach has been reported on the preparation of graphene decorated with magnetic Fe3O4 nanoparticles for immobilizing porcine pancreatic lipase. The microstructures and interaction of the hybrids were characterized by several methods, which indicated that the Fe3O4 nanoparticles are chemically bonded onto graphene sheets with tunable size and density. The graphene/Fe3O4 hybrids provide excellent efficiency in immobilizing porcine pancreatic lipase, though dependent on pH value, temperature and denaturant. The porcine pancreatic lipase immobilized on graphene/Fe3O4 hybrids becomes recyclable and have a remarkable thermal-stability in comparison with a free porcine pancreatic lipase. Our work demonstrates that graphene/Fe3O4 hybrids could be high-quality and novel carriers for immobilizing lipase, which could be a good candidate for lipase immobilization. (C) 2013 Elsevier Ltd. All rights reserved.