▎ 摘　　要

The rapid rise of cancer worldwide demonstrates the importance of treatment strategies. In addition to reduce the side effects of conventional treatments, targeted drug delivery systems increase performance and effectiveness. In this research, nanocarriers comprising gelatin (G)-polyvinylpyrrolidone (PVP) coated graphene oxide (GO) were prepared for the first time. The nanocarries were loaded with quercetin (QC) drug and a dual nanoemulsion water/oil/water with bitter almond oil was developed as a membrane around the nanocomposite to control further drug release. XRD, FTIR, FESEM, and DLS analysis confirmed the success of the nanocomposite synthesis and drug loading. The resulting pH-sensitive drug delivery system showed an 87.5% encapsulation efficiency and a 45% drug loading, which are among the highest values reported up to date. The zeta potential of the nanocomposites was about -40 mV, indicating good stability. The release kinetics of the drug followed the Higuchi model, and the presence of a dual nanoemulsion resulted in better drug entrapping efficiency, drug controlled release and long-term release. MTT assay and flow cytometry methods revealed a rate of cancer cell death of 53.14%, which was 36.51% in the apoptotic phase. Taking into account the results obtained herein, PVP-G-GO-QC can be considered as a new promising system for cancer treatment.