▎ 摘　　要

We first reported a facile method to prepare porous graphene with tunable structure for glucose oxidase (GOD) immobilization based on freeze-drying. The produced porous graphene (PGR) exhibited three-dimensional interconnected porous structure. Moreover, their microstructure was easily tuned by addition of surfactant and oil droplets into the graphene oxide dispersion. Field emission scanning electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, Raman spectroscopy and nitrogen physisorption were used to characterize microstructures, composition and surface area of the produced PGR. The addition of surfactant introduced mesopores in the PGR and the oil droplets can improve the pore volume. It was found that the PGR structure obviously affected the electrochemical behavior of GOD. Electrocatalytical activity towards glucose was also investigated, and the maximum electrocatalytical current reached 6.3 times for 5 mM glucose. The modified electrode was used to detect the glucose, and a low detection limit of 8.7 OA and a high sensitivity of 16.3 pAmM cm(-2) were obtained. The good sensing performance was attributed to the good three-dimensional porous structure and conductivity of the PGR. (C) 2016 Elsevier B.V. All rights reserved.