▎ 摘　　要

Detection of hydrogen peroxide (H2O2) is very important in biological analysis, clinical diagnosis, food industry, etc. In this work, we develop a new sensor based on immobilization of Cytochrome c (Cyt c) on graphene aerogel/platinum nanoparticle (GA/Pt wNPs) nanocomposites for the detection of H2O2. This H2O2 sensor exhibited good sensitivity (5.3 mu A . mM(-1) . cm(-2)), a wider linear detection range of H2O2 concentration (5 mu mol . L-1 to 1175 mu mol . L-1), and a low detection limit of 1.67 mu mol.L-1, as well as good stability and selectivity. The excellent performance of the sensor is attributed to large specific surface area and high conductivity of graphene and Pt NPs, and good biocompatibility of Nafion, which improve the enzyme absorption and promote direct electron transfer between Cyt c and the electrode surface. These results indicate that GA/Pt NPs composite provides a new avenue for immobilizing proteins and fabricating excellent biosensors.