▎ 摘　　要

By using graphene as a spacer, multilayer ultrathin films composed of Prussian blue nanoparticles and glucose oxidase have been fabricated on a glass carbon electrode via electrostatic layer-by-layer (LbL) assembly technique to construct glucose biosensors. This work demonstrated the capability of graphene as the spacer to form electrochemically functionalized inorganic and organic hybrid nanoarchitecture. The buildup and electrochemical properties of the multilayer nanoarchitecture were investigated by UV-Vis spectroscopy, atomic force microscopy technique and cyclic voltammetry, respectively. It was found that the developed glucose biosensor allowed rapid and sensitive determination of glucose. The amperometric response exhibited a linear correlation to glucose concentration over a range from 0.1 to 6.5 mM and the response time and detection limit were determined to be 2 s and 6 mu M, respectively. It can be expected that the combination of useful graphene and versatile LbL technique could develop more efficient and more advanced biosensing devices.