• 文献标题:   A layered nanocomposite of laccase, chitosan, and Fe3O4 nanoparticles-reduced graphene oxide for the nanomolar electrochemical detection of bisphenol A
  • 文献类型:   Article
  • 作  者:   FERNANDES PMV, CAMPINA JM, SILVA AF
  • 作者关键词:   graphene nanomaterial, graphenenanoparticle conjugate, magnetite nanoparticle, enzymatic biosensor, fesem, xps, atrftir, cyclic voltammetry, square wave voltammetry, electrochemical impedance spectroscopy, chronoamperometry
  • 出版物名称:   MICROCHIMICA ACTA
  • ISSN:   0026-3672 EI 1436-5073
  • 通讯作者地址:   Univ Porto CIQUP
  • 被引频次:   3
  • DOI:   10.1007/s00604-020-4223-x
  • 出版年:   2020

▎ 摘  要

A hybrid conjugate of reduced graphene oxide/ferrous-ferric oxide nanoparticles (rGO-Fe3O4 NPs) is characterized and assembled with chitosan and laccase to form a layered functional superstructure. After its characterization by field-effect scanning electron microscopy, energy-dispersive X-ray analysis, X-ray photoelectron spectroscopy, attenuated total reflectance Fourier transform infrared, cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS), the nanocomposite has been deposited on glassy carbon for the enzyme-mediated electrochemical determination of the endocrine disruptor bisphenol A (BPA). Proof-of-concept assays conducted by using CV, EIS, and square wave voltammetry reveal that the enzymatic biosensor provides linear response in a wide range of BPA concentrations (6-228 ppb), very high sensitivities, and excellent durability (over 1-month storage). Using amperometric detection, remarkable sensitivities (2080 mu A mu M-1 cm(-2)) and detection limits (18 nM) are attained. Applications to real samples of bottled water proved feasible with recoveries in the range 107-124%.