• 文献标题:   Sandwich electrochemical thrombin assay using a glassy carbon electrode modified with nitrogen- and sulfur-doped graphene oxide and gold nanoparticles
  • 文献类型:   Article
  • 作  者:   HE BS
  • 作者关键词:   carbon material, protein, aptamer, signal amplification
  • 出版物名称:   MICROCHIMICA ACTA
  • ISSN:   0026-3672 EI 1436-5073
  • 通讯作者地址:   Henan Univ Technol
  • 被引频次:   7
  • DOI:   10.1007/s00604-018-2872-9
  • 出版年:   2018

▎ 摘  要

Graphene oxide doped with nitrogen and sulfur was decorated with gold nanoparticles (AuNP-SN-GO) and applied as a substrate to modify a glassy carbon electrode (GCE). An aptamer against the model protein thrombin was self-assembled on the modified GCE which then was exposed to thrombin. Following aptamer-thrombin interaction, biotin-labeled DNA and aptamer 2 are immobilized on another AuNP-SN-GO hybrid and then are reacted with the thrombin/AuNP-SN-GO/GCE to form a sandwich. The enzyme label horseradish peroxidase (HRP) was then attached to the electrode by biotin-avidin interaction. HRP catalyzes the oxidation of hydroquinone by hydrogen peroxide. This generates a strong electrochemical signal that increases linearly with the logarithm of thrombin concentration in the range from 1.0 x 10(-13) M to 1.0 x 10(-8) M with a detection limit of 2.5 x 10(-14) M (S/N = 3). The assay is highly selective. It provides a promising strategy for signal amplification. In our perception, it has a large potential for sensitive and selective detection of analytes for which appropriate aptamers are available.