▎ 摘　　要

Here, we present an electrochemical biosensor for sensitive, specific, and rapid detection of lung cancer biomarker, neuron-specific enolase by utilization of rGO-modified r-MoS2 multi-layered nanosheets as a matrix. Layered structures along with the synergetic effect of r-MoS2 and rGO allow easy grafting of two materials into one another. This empowers the sensor to have a heterogeneous rate transfer constant of 1.29 x 10(-3) cm/s and an electroactive surface area of 27.83 mm(2). Electrochemical impedance spectroscopy and cyclic voltammetry are used as dual modes for the detection of NSE, and the sensor exhibits a wide linear detection range. The sensor is found to be reproducible with a relative standard deviation of less than 5% along with an invariant response towards other endogenous interfering species found in human serum. The investigations done in this study will further help us to fabricate a device for rapid and early detection of lung cancer.