• 文献标题:   ROS Scavenging Graphene-Based Hydrogel Enhances Type H Vessel Formation and Vascularized Bone Regeneration via ZEB1/Notch1 Mediation
  • 文献类型:   Article
  • 作  者:   ZHOU JP, LI YW, HE JH, LIU LY, HU SG, GUO M, LIU T, LIU JZ, WANG JX, GUO BL, WANG W
  • 作者关键词:   bone regeneration, reduced graphene oxide rgo, reactive oxygen species ros, type h vessel
  • 出版物名称:   MACROMOLECULAR BIOSCIENCE
  • ISSN:   1616-5187 EI 1616-5195
  • 通讯作者地址:  
  • 被引频次:   0
  • DOI:   10.1002/mabi.202200502 EA JAN 2023
  • 出版年:   2023

▎ 摘  要

The regeneration strategy for bone defects is greatly limited by the bone microenvironment, and excessive reactive oxygen species (ROS) seriously hinder the formation of new bone. Reduced graphene oxide (rGO) is expected to meet the requirements because of its ability to scavenge free radicals through electron transfer. Antioxidant hydrogels based on gelatine methacrylate (GM), acrylyl-beta-cyclodextrin (Ac-CD), and rGO functionalized with beta-cyclodextrin (beta-CD) are developed for skull defect regeneration, but the mechanism of how rGO-based hydrogels enhance bone repair remains unclear. In this work, it is confirmed that the GM/Ac-CD/rGO hydrogel has good antioxidant capacity, and promotes osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and angiogenesis of human umbilical vein endothelial cells (HUVECs). The rGO-based hydrogel affects ZEB1/Notch1 to promote tube formation. Furthermore, two-photon laser scanning microscopy is used to observe the ROS in a skull defect. The rGO-based hydrogel promotes type H vessel formation in a skull defect. In conclusion, the hydrogel neutralizes ROS in the vicinity of a skull defect and stimulates ZEB1/Notch1 to promote the coupling of osteogenesis and angiogenesis, which may be a possible approach for bone regeneration.