▎ 摘　　要

A combined method of electrostatic adsorption and green reduction was successfully employed to synthesize graphene-loaded carbon nanofibers (G/CNFs). The graphene nanoflakes enhanced the degree of graphitization of CNFs. A mixture containing laccase (Lac), G/CNFs and Nafion was used to fabricate a novel biosensor. The G/CNFs facilitated the direct electron transfer between Lac and the modified electrode. A pair of apparently enhanced redox peaks was observed on the modified electrode. The biosensor was investigated using square wave voltammetry, and the biosensor displayed highly efficient electrocatalysis towards catechol with a detection limit of 20 nM (S/N = 3), a sensitivity of 13.5 mu A mu M-1, and a linear range from 1 x 10(-7) to 2.86 x 10(-6) M. Moreover, this new biosensor also showed good stability and selectivity, and was employed in measuring catechol concentration in a real water environment successfully.