▎ 摘　　要

Tyrosine is a non-essential amino acid that presents in most fluids and all tissues in the human body and plays a crucial role in human body activities. Herein, an enzymatic biosensor based on the modification of glassy carbon electrode (GCE) with tyrosine hydroxylase (TryHAS) and reduced graphene oxide (rGO) fabricated and utilized to monitor tyrosine in food samples. The rGO/TryHAS/GCE offered powerful catalytic activity for sensing tyrosine with a linear dynamic range of 0.001-345 mu M, and a detection limit of 0.7 nM. Furthermore, the selectivity of rGO/TryHAS/GCE was assessed for monitoring tyrosine in an aqueous solution, and the findings revealed a high selectivity in the sensing process. In the final step, rGO/TryHAS/GCE was utilized for the determination of tyrosine in real food samples including egg and cheese, with acceptable recovery data.