▎ 摘　　要

A three-dimensional reduced graphene oxide (3D GR)-Prussian blue (PB)-based electrochemical enzyme sensor was constructed for sensitive detection of uric acid. The graphene oxide was thermally reduced by one-step hydrothermal method, and then 3D GR was formed by self-assembly. PB was electrodeposited onto 3D GR to synthesize 3D GR-PB composite. Due to its large specific surface area and porous nature, 3D GR provided more active sites for the reaction. Electrochemical tests showed that the enzyme sensor based on 3D GR-PB had a wide linear range (3.75 x 10(-9) - 3.75 x 10(-4) mol/L) and a low detection limit (4.21 x 10(-10) mol/L). Because of the high specificity of uricase to catalyze uric acid, this sensor had high selectivity and provided a feasible solution for sensitive detection of uric acid.