• 文献标题:   An amperometric zearalenone aptasensor based on signal amplification by using a composite prepared from porous platinum nanotubes, gold nanoparticles and thionine-labelled graphene oxide
  • 文献类型:   Article
  • 作  者:   HE BS, YAN XH
  • 作者关键词:   aptamer, insitu synthesi, electrodeposited gold nanoparticle, rapid detection, low detection limit
  • 出版物名称:   MICROCHIMICA ACTA
  • ISSN:   0026-3672 EI 1436-5073
  • 通讯作者地址:   Henan Univ Technol
  • 被引频次:   11
  • DOI:   10.1007/s00604-019-3500-z
  • 出版年:   2019

▎ 摘  要

The authors constructed a voltammetric zearalenone (ZEN) aptasensor based on use of porous platinum nanotubes, gold nanoparticles (p-PtNTs/AuNPs) and thionine (Thi) labelled graphene oxide (GO). The p-PtNTs were synthesized in-situ based on tellurium nanowires as sacrificial templates. Subsequently, thiol-modified aptamers were self-assembled on the AuNPs that had been electrodeposited on the surface of the modified electrode. The presence of p-PtNTs on the electrode increases the loading with AuNPs and aptamers. It also warrants that the Thi-labelled GO can be assembled onto the aptamer via interactions. In the presence of ZEN, it will be bound by the aptamer. The GO/Thi conjugate will be released from the aptamer, and this causes a decrease in Thi current. Under the optimal conditions and at a typical working potential of -0.22V (vs. Ag/AgCl), the method has a linear range that covers the 0.5pgmL(-1) to 0.5gmL(-1) ZEN concentraion range and a lower detection limit of 0.17pgmL(-1).