▎ 摘　　要

We investigate the effect of cell culture conditions, using pristine graphene sheets as growth substrate, on the human nerve cell line (SH-SY5Y). The cell viability and morphology were evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), lactate dehydrogenase (LDH) and caspase-3/7 activity assays, as well as fluorescence microscopy of cells stained with Hochest 33342 and Calcein AM. Human nerve cells exhibited 84% viability on pristine graphene sheets compared with control (cell culture polystyrene) after 3 days culturing. However, pristine graphene hardly induced apoptosis and allowed for 92% viability on necrosis, compared to positive control. Fluorescence data showed that the presence of graphene did not influence cell morphology. These results suggest that graphene sheets can be applied to advance tissue culture techniques for facilitating tissue repair and regeneration.