▎ 摘　　要

A monolayer graphene electrochemical platform based on chemical vapor deposition grown graphene was constructed for electrochemical detection of nicotinamide adenine dinucleotide (NADH). Caffeic acid (CFA) was immobilized on the surface of the activated graphene platform electrode (GPE) via electrodeposition. The CFA-GPE was characterized by scanning electron microscopy, atomic force microscopy, energy dispersive spectrometer, X-ray photoelectron spectroscopy, Raman spectra, and Fourier transform infrared spectra. The electrochemical behaviors of NADH were investigated by cyclic voltammetry. With the synergetic effect of graphene and CFA, the overpotential of NADH oxidation peak decreased by 0.43 V and its peak current increased significantly. Hence, a novel electrochemical sensor for selective detection of NADH was created. The broad linear response was presented by chronoamperometry, which was 0.001 mu mol/L to 1200 mu mol/L with a detection limit of 0.52 mu mol/L (S/N = 3). The simultaneous determination of NADH, glutathione and folic acid was realized by differential pulse voltammetry technique at CFA-GPE. In addition, the as-obtained sensor delivered good stability as well as high resistance to interference, and was successfully utilized in real sample detection.