▎ 摘　　要

Tri-layered reduced graphene oxide with better graphitization was synthesized and functioned using radio frequency N-2 and O-2 plasma. The layer numbers of reduced graphene oxide were determined by atomic force microscopy (AFM) and x-ray diffraction (XRD). The effect of plasma treatment on crystal structure, surface morphology and chemical composition were studied from XRD, transmission electron microscopy (TEM), x-ray photoelectron spectroscopy (XPS), Fourier transforms infrared spectroscopy (FTIR) and Raman spectroscopy. The chemical species present in N-2/O-2 plasma during functionalization of tri-layered reduced graphene oxide was analyzed by optical emission spectroscopy. Tri-layered reduced graphene oxide and functioned tri-layered reduced graphene oxide exhibits higher electrochemical performance towards ferrocyanide redox reaction than glassy carbon and platinum electrode with much decrease in overpotential. This indicates that tri-layered reduced graphene oxide and N2/O2 functionalized tri-layered reduced graphene oxide are promising working electrodes in the application of electrochemical based biosensor.