▎ 摘　　要

Detection of the fusion gene composed of echinoderm microtubule-associated protein-like 4 (EML4) and anaplastic lymphoma kinase (ALK) is important for diagnosis of lung adenocarcinoma. We developed a simple PCR-based fluorometric method for detecting the EML4-ALK fusion using a fluorescent probe DNA and graphene oxide (GO). The fluorophore-labeled probe DNA complementary to the EML4-ALK fusion junction was hydrolyzed during PCR by the 5 ' to 3 ' exonuclease activity of the Taq DNA polymerase. The fluorophore released from the probe DNA retained fluorescence without adsorption onto GO. Our GO-based fluorometric system was able to detect a single cancer cell harboring the EML4-ALK fusion in 10(6) wild-type cells.