▎ 摘　　要

A nonmagnetically modified graphene was employed as the adsorbent in the dispersive solid-phase extraction (d-SPE) for the isolation of permethrin from urine samples that were further analyzed by the HPLC/UV method. The following four key factors influencing permethrin extraction efficacy were studied: 1. graphene suspension volume, 2. adsorption time, 3. desorbent (acetonitrile) volume, and 4. desorption time. Under the optimized conditions of the d-SPE (graphene) procedure the main validation parameters of the analytical method were determined as follows: extraction recovery (%) 71.2, 63.5, and 95.4; intra-day repeatability (%RSD) 14.7, 11.5, and 15.2; inter-day repeatability (%RSD) 16.2, 11.9, and 19.1 for 0.125, 0.250, and 1.0 mu g/mL permethrin concentration levels, respectively. Limits of detection (LOD) and quantification (LOQ) were 0.045 and 0.125 mu gmL(-1), respectively. The proposed method may be useful for the monitoring of permethrin in human urine taken from people dressed in uniforms disinfected with this pesticide.