▎ 摘　　要

Here, we report a non-enzymatic Silver nanoparticle-decorated graphene oxide (Ag NPs-GO) nanocomposite based electrochemical biosensor for the detection of ascorbic acid using the ultra-sensitive electrochemical method. In this work, Ag NPs-GO nanocomposite synthesized through simple and cost-effective ultra-sonication method. As produced composite was characterized by FE-SEM, TEM, EDX, FT-IR, XRD, EIS and electrochemical methods. The electrochemical behavior of ascorbic acid at the surface of Ag NPs-GO nanocomposite modified screen-printed carbon electrode (SPCE) was investigated by cyclic voltammetry (CV) technique while ascorbic acid sensing was performed by Differential pulse voltammetry method. The as-developed biosensor exhibited lower detection limit of 25 nM and the sensitivity is 1.71 mu A mu M-1 cm(-2) (R-2=0.997) towards ascorbic acid in the linear range of 1-210 mu M with no surface fouling was observed. To evaluate the practical application, the detection of AA performed in the real sample of human urine and vitamin C tablets to predict unknown concentrations of in the PBS pH 7.0.