▎ 摘　　要

Surface modifications of titanium (Ti) to improve the potential of implantable materials for orthopedic applications are widely studied. Coating the surface of a biomaterial with graphene oxide (GO) increases the durability of orthopedic implants due to the biocompatibility and antibacterial properties of GO. In the present study, anodized titanium (ATi) was coated with GO via anodization of Ti and anodic-electrophoretic deposition of GO at 200 mu g/ml (ATiGO200). Cell culture results suggested that the surface topographic features of ATi and GO coating act synergistically to improve synthesis of the bone matrix and mineralization. Total protein content, calcium deposition, and alkaline phosphatase activity assays confirmed that osteogenic differentiation was improved on ATiGO200. Expression of the bone matrix protein genes (Bglap and Alpl) was upregulated on ATiGO200 during late-stage osteoblast differentiation. Combined anodization of Ti and electrodeposition of GO provides a bioactive surface for osteogenic differentiation to enhance osseointegration of orthopedic implants.