▎ 摘　　要

In this study, we have for the first time preformed the facile substrate-enhanced electroless deposition (SEED) of metal nanoparticles onto monolithic graphene@Ni foams for construction of disposable three-dimensional (3D) electrochemical immunosensors. Specifically, we firstly used the SEED method to deposit gold nanoparticles (AuNPs) onto the graphene@Ni foam for immobilization of antibody (Ab(1)). This is followed by a second step SEED deposition to produce silver nanoparticles (AgNPs) for electrochemical stripping detection. Using alpha-fetoprotein antigen (AFP) as a module analyte, the newly-developed sensor showed a wide linear response, ranging from 5.0 pg/mL to 5.0 ng/mL and a low detection limit down to 2.3 pg/mL. The newly-developed 3D-immunosensor is sensitive, reliable, and easy to be fabricated, showing great potential for clinic applications. (C) 2019 Science China Press. Published by Elsevier B.V. and Science China Press. All rights reserved.