▎ 摘　　要

Graphene oxide nanoparticles (nGOs) were prepared by nanoscond pulsed laser ablation (ns-PLA) of graphene solution at a wavelength of 1064 nm. The High-Resolution Transmission Electron Microscopy (HR-TEM) reveals the average particles size of nGOs as 26 nm with an interplanar spacing of 0.21-0.24 nm indicating the crystalline nature of nGOs. X-ray Diffraction (XRD) analysis exhibited peak at 14.4 degrees suggesting the exfoliation of graphene sheet under the PLA of graphene solution. Raman, Fourier Transform Infrared Spectroscopy (FTIR) and X-ray Photoelectron Spectroscopic (XPS) analysis reveals the oxidation of graphene precursor. Photoluminescence (PL) studies of nGOs showed the excitation-dependent PL emission with peak PL emission intensity in the bluish-green region (455 nm). In-vitro studies indicate that synthesized nGOs at a low and high concentration (1, 5 and 10% v/v) showed no direct cytotoxicity on smooth muscle cells at different time points. Moreover, they do not affect the proliferation rate of smooth muscles cells. Herein, nGOs prepared by PLA was found to be photoluminescent and biocompatible, which can be utilized for bioimaging applications.