▎ 摘　　要

Here, we examined the effects of reduced graphene oxide (rGO) nanosheets-covered hydroxyapatite (HA) nanocomposites (rGO/HA NCs) on the osteodifferentiation of bone marrow-derived mesenchymal stem cells (BMSCs) and compared their effects according to the culture conditions with and without osteogenic inducers. The alkaline phosphatase activity and calcium deposition were determined as early and late stage markers of osteodifferentiation of BMSCs. Our results indicate that the rGO/HA NCs stimulate the spontaneous osteogenesis in BMSCs cultured without osteoinductive agents and their osteogenic activity is further enhanced when those agents are added to the culture media. This suggests that rGO/HA NCs can be exploited to develop strategies for the development of novel dental and orthopedic bone grafts since these graphene-related composite materials have potent effects on stimulating osteogenesis and show superior bioactivity and osteoinductive potential.