• 文献标题:   Impedimetric Tumor Necrosis Factor-alpha Sensor Based on a Reduced Graphene Oxide Nanoparticle-Modified Electrode Array
  • 文献类型:   Article
  • 作  者:   YAGATI AK, LEE GY, HA S, CHANG KA, PYUN JC, CHO S
  • 作者关键词:   graphene oxide, nanoparticle, tumor necrosis factoralpha, impedance, biosensor
  • 出版物名称:   JOURNAL OF NANOSCIENCE NANOTECHNOLOGY
  • ISSN:   1533-4880 EI 1533-4899
  • 通讯作者地址:   Gachon Univ
  • 被引频次:   8
  • DOI:   10.1166/jnn.2016.13618
  • 出版年:   2016

▎ 摘  要

A surface was prepared by simple electrodeposition of reduced graphene oxide (RGO) coated with gold nanoparticles (AuNP) on an indium tin oxide microelectrode array, and its ability and applicability in a tumor necrosis factor-alpha (TNF-alpha) detection sensor was evaluated by electrochemical impedance spectroscopy (EIS). The AuNP-RGO hybrid structure showed improved electrical conductivity when compared with the conductivities of its individual elements and demonstrated an excellent capturing ability for TNF-alpha antibody-antigen binding. A quantitative determination of the TNF-alpha detection performance was achieved by measuring the resistance changes with varying TNF-alpha concentrations while using a [Fe(CN)(6)](3-/4-) redox probe in EIS. The antibody covered electrode resistance increases approximately linearly (r = 0.969) with increasing antigen concentration. The proposed TNF-alpha sensor has a 0.43 pg mL(-1) minimum detection limit and a linear range of 1-1000 pg mL(-1).