• 文献标题:   Novel Graphene Biosensor Based on the Functionalization of Multifunctional Nano-bovine Serum Albumin for the Highly Sensitive Detection of Cancer Biomarkers
  • 文献类型:   Article
  • 作  者:   ZHOU L, WANG K, SUN H, ZHAO SM, CHEN XF, QIAN DH, MAO HJ, ZHAO JL
  • 作者关键词:   biointerface, multifunctional denatured bsa, gfet biosensor, cancer biomarker
  • 出版物名称:   NANOMICRO LETTERS
  • ISSN:   2311-6706 EI 2150-5551
  • 通讯作者地址:   Chinese Acad Sci
  • 被引频次:   6
  • DOI:   10.1007/s40820-019-0250-8
  • 出版年:   2019

▎ 摘  要

A simple, convenient, and highly sensitive bio-interface for graphene field-effect transistors (GFETs) based on multifunctional nano-denatured bovine serum albumin (nano-dBSA) functionalization was developed to target cancer biomarkers. The novel graphene-protein bioelectronic interface was constructed by heating to denature native BSA on the graphene substrate surface. The formed nano-dBSA film served as the cross-linker to immobilize monoclonal antibody against carcinoembryonic antigen (anti-CEA mAb) on the graphene channel activated by EDC and Sulfo-NHS. The nano-dBSA film worked as a self-protecting layer of graphene to prevent surface contamination by lithographic processing. The improved GFET biosensor exhibited good specificity and high sensitivity toward the target at an ultralow concentration of 337.58fgmL(-1). The electrical detection of the binding of CEA followed the Hill model for ligand-receptor interaction, indicating the negative binding cooperativity between CEA and anti-CEA mAb with a dissociation constant of 6.82x10(-10)M. The multifunctional nano-dBSA functionalization can confer a new function to graphene-like 2D nanomaterials and provide a promising bio-functionalization method for clinical application in biosensing, nanomedicine, and drug delivery.