▎ 摘　　要

In this study, graphene oxide (GO)-incorporated poly(lactic-co-glycolic acid, PLGA)-collagen (Col) fibrous matrices were fabricated by electrospinning and analyzed for tissue engineering applications to biomimetic scaffolds. The physicochemical properties of GO-incorporated PLGA-Col fibrous matrices were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), Raman spectroscopy and thermogravimetric analysis (TGA). SEM and AFM images showed that GO-incorporated PLGA-Col fibrous matrices were successfully fabricated and have a suitable structure for tissue engineering scaffolds. The diameter of GO-incorporated PLGA-Col fibers was found to range between 300 and 900 nm. Raman spectra revealed that the GO was well incorporated in the hybrid fibers. TGA profiles demonstrated that the decomposition and weight loss of the fibrous matrices were progressed in the temperature about 300 degrees C. From this result, fabricated matrices are stable in the cell culture condition. Moreover, the initial attachment and proliferation of primarily cultured vascular smooth muscle cells (VSMCs) were increased on GO-incorporated PLGA-Col fibrous matrices. These results suggested that GO-incorporated PLGA-Col hybrid matrices can enhance the cellular behaviors including initial attachment and proliferation. Therefore, GO-incorporated PLGA-Col fibrous matrices are beneficial to the growth of primary cells and they have potentials to serve as biomimetic scaffolds.