• 文献标题:   Gold-coated graphene field-effect transistors for quantitative analysis of protein-antibody interactions
  • 文献类型:   Article
  • 作  者:   TARASOV A, TSAI MY, FLYNN EM, JOINER CA, TAYLOR RC, VOGEL EM
  • 作者关键词:   graphene fieldeffect transistor, potentiometric biosensor, protein interaction
  • 出版物名称:   2D MATERIALS
  • ISSN:   2053-1583
  • 通讯作者地址:   Georgia Inst Technol
  • 被引频次:   16
  • DOI:   10.1088/2053-1583/2/4/044008
  • 出版年:   2015

▎ 摘  要

Field-effect transistors (FETs) based on large-area graphene and other 2D materials can potentially be used as low-cost and flexible potentiometric biological sensors. However, there have been few attempts to use these devices for quantifying molecular interactions and to compare their performance to established sensor technology. Here, gold-coated graphene FETs are used to measure the binding affinity of a specific protein-antibody interaction. Having a gold surface gives access to well-known thiol chemistry for the self-assembly of linker molecules. The results are compared with potentiometric silicon-based extended-gate sensors and a surface plasmon resonance system. The estimated dissociation constants are in excellent agreement for all sensor types as long as the active surfaces are the same (gold). The role of the graphene transducer is to simply amplify surface potential changes caused by adsorption of molecules on the gold surface.