▎ 摘　　要

The emerging demand for antibiofilm coating nanostructures has led to the design of graphene oxide (GO) hosted copper nanoclusters (CuNCs). In this study, we have developed red fluorescence emitting Cu@GO@CTAB nanostructures. The size of the CuNCs in Cu@GO@CTAB was found to be similar to 16.7 +/- 2.1 nm. The developed nanoassembly showed dual fluorescence emission maxima at similar to 474 and similar to 645 nm and revealed more promising antibiofilm potential for E. coli (MBIC50 similar to 74.1 +/- 5.1 mu g mL(-1)) as compared to S. aureus. Interestingly, nanomaterials (NMs) have shown ratiometric interaction towards E. coli isolated extracellular matrix fibrils wherein an isoemissive point was obtained at similar to 569 nm. The limit of detection for extracellular matrix fibrils from E. coli and lipopolysaccharide (LPS) was found to be similar to 331.1 +/- 12.3 and 9.47 +/- 1.12 mu g mL(-1), respectively. Confocal studies suggested that Cu@GO@CTAB NMs can preferentially penetrate and reduce the overall thickness of E. coli biofilm. Motility assays suggested that the developed nanostructures restricted the motility of E. coli and S. aureus. Mechanistic assays for outer and inner membranes suggested that bacterial membrane disruption induced cell death. A displacement assay using BODIPY TR cadaverine (BC) revealed that the prepared NM showed strong binding to LPS of E. coli O26:B6. These studies may pave the path for developing antibacterial and antibiofilm coating agents.