▎ 摘　　要

Cardiotoxicity and other side effects of current antineoplastic agents with narrow therapeutic windows, such as doxorubicin hydrochloride (DOX), have increased the need for advanced real-time monitoring approaches to control their dosage and minimize common side effects. In this study, we reinforced the biocompatible and electrosensitive polymeric structure of polyrhodanine with iron(II,III) oxide (Fe3O4) and coated graphene oxide (GO) with Fe3O4 (GO-Fe3O4) nanoparticles. The resulting hybrid polymeric platform was bio-enhanced by extraction of kombucha solvent supernatant and used as a highly sensitive biosensor with enhanced electroactive C=N+ functional groups for real-time detection of DOX in the human blood plasma sample. The developed platform was well characterized by performing different analyses, and its applicability was evaluated using an electrochemical approach. The results showed the superior sensitivity (167.62 mu A mu M-1 cm(-2)), low detection limit (DL, 0.008 mu M), and quantification limit (QL, 0.056 mu M) of the proposed biosensor. The biosensor detected DOX in the biological fluid with optimal accuracy (>99%), highlighting the ideal performance of the fabricated polymeric biosensor.