▎ 摘　　要

In this report, a sensitive and selective chemiluminescence (CL) biosensor for bovine serum albumin (BSA) coupled with a surface molecularly imprinted polymer nanocomplex using beta-cyclodextrin/chitosanmagnetic graphene oxide as backbone material (beta-CD/Cs-MGO-SMIP) was investigated. The material beta-CD/Cs-MGO combined with beta-cyclodextrin, chitosan and graphene oxide was used to provide multiple imprinting sites and a large surface area was characterized by SEM, XRD and FTIR. It was found that beta-CD/Cs-MGO-SMIP followed the Langmuir isotherm equation and pseudo-second order sorption kinetics when binding the template. This material demonstrated fast mass transfer, a promoted rate of removal of the biomacromolecule and excellent recognition and adsorption ability for the imprinting cavities situated at the surface of beta-CD/Cs-MGO, which enabled easy access to BSA. Subsequently, a highly sensitive CL biosensor for BSA was proposed based on the strong recognition effect between beta-CD/Cs-MGO-SMIP and BSA which led to a high selectivity of the sensor, and the proposed biosensor could assay in the range 5.0 x 10(-7) to 1.0 x 10(-4) mg mL(-1) with a detection limit of 1.1 x 10(-7) mg mL(-1). The obtained recoveries were between 94% and 106% when determining samples.