▎ 摘　　要

Heterogeneous template-induced nucleation is a promising way to regulate protein crystallization events and could be employed for purification processes and crystallographic studies. Protein crystallization process with graphite and graphene oxide, as heterogeneous templates, were investigated. More than 640 hanging drops with different concentrations of Lysozyme (30, 50, 70,100 mg/mL) and NaCl (0.7, 0.9, 1.1, 1.3, 1.5 M) were crystallised at 4 degrees C with or without graphite/graphene oxide templates. The induction times and crystallization process were observed under the microscope. The lysozyme in the solutions with graphite flakes nucleated faster under all the conditions than the lysozyme with equal experimental conditions without templates. The crystals preferred to grow around the edge of graphite flakes than on the flat surfaces. In the droplets with monolayer graphene oxide, more crystals appeared around graphene oxide particles, and the faster or slower nucleation processes with templates were dependent on the lysozyme and NaCl concentrations. Graphene oxide templates strongly inhibited nucleation at high lysozyme concentrations but promoted nucleation at low lysozyme concentrations. Both heterogeneous templates changed the crystal morphology and the crystallization kinetics. More crystals were observed in the solution with graphite templatesthan with graphene oxide templates and without any template.(c) 2022 Chinese Society of Particuology and Institute of Process Engineering, Chinese Academy of Sciences. Published by Elsevier B.V. This is an open access article under the CC BY license (http:// creativecommons.org/licenses/by/4.0/).