• 文献标题:   Aerogels prepared from polymeric beta-cyclodextrin and graphene aerogels as a novel host-guest system for immobilization of antibodies: a voltammetric immunosensor for the tumor marker CA 15-3
  • 文献类型:   Article
  • 作  者:   JIA HY, TIAN QY, XU JK, LU LM, MA X, YU YF
  • 作者关键词:   betacyclodextrin polymer, threedimensional structure, biosensor, carbohydrate antigen 153, immunoassay, hostguest immobilization, breast cancer, human serum, biomarker
  • 出版物名称:   MICROCHIMICA ACTA
  • ISSN:   0026-3672 EI 1436-5073
  • 通讯作者地址:   Jiangxi Sci Technol Normal Univ
  • 被引频次:   5
  • DOI:   10.1007/s00604-018-3056-3
  • 出版年:   2018

▎ 摘  要

A three-dimensional porous network graphene aerogel (GAs) with large specific area and excellent conductivity was loaded with ss-cyclodextrin polymer (P ss-CD) to serve as a support for immobilization of antibodies. A highly sensitive immunosensor for the cancer marker carbohydrate antigen 15-3 (CA15-3) was designed based on the use of P ss-CD/ GAs. The large specific area of GAs warrants high loading with antibodies, and their excellent electrical conductivity warrants strong electrical signals. Based on the synergistic effect of GAs and P ss-CD, an immunoassay was designed that is making use of hexacyanoferrate as an electrochemical probe and having a pleasantly low working potential of 0.2 V (vs. SCE). Response is linear in the 0.1 mU mL-1 to 100 U mL-1 activity range, and the lower detection limit is 0.03 mU mL-1 (at S/ N = 3). The immunoassay is stable, selective and reproducible. It was applied to the analysis of spiked samples, and results were satisfactory.