▎ 摘　　要

Magnetic graphene oxide (MGO) was prepared using graphene oxide (GO) and Fe3O4 . Chloroperoxidase (CPO) was immobilized via adsorption method in MGO, and the immobilization conditions , such as the temperature , time and pH value of the buffer solution, were optimized. The operating stability of immobilized CPO was studied using CPO as catalyst for the degradation of acid blue 45. The activity stability of immobilized CPO was investigated from four aspects of acid base stability, H2O2 stability, thermal stability and storage stability using CPO as catalyst for the decolorization of acid blue 45. The results show that the optimal conditions are the phosphate buffer pH = 3. 5, the reaction time 15 min and the temperature 15 degrees C . TMGO {m[NH4Fe(SO4)(2) center dot 12H(2)O] : m(GO) = 10.7 : 1} is a better immobilized carrier than FMGO {m[NH4Fe(SO4)(2) center dot 12H(2)O] : m (GO) = 5. 35 : 1} . And the immobilized CPO shows a better operation stability than the free enzyme. The accumulation layer of TMGO is opened at 35-50 degrees C , leading to the loss rate of immobilized enzyme activity significantly less than that of the free enzyme. TMGO-CPO has a good reusability that the activity is still over 60% after recycling 5 times.