▎ 摘　　要

Nano- and hybrid materials have recently emerged as a new approach for improving enzyme activity and stability, and are suitable for commercial applications. In this paper, graphene oxide-based magnetic hybrids were prepared successfully. A chloropropyl-functionalized graphene oxide decorated with Fe3O4 nanoparticles was made, denoted as CPS/GO-Fe3O4@ MCM-41. This was characterized by X-ray powder diffraction, scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, vibrating sample magnetometry, thermogravimetry and N-2 adsorption/desorption. Then, porcine pancreas lipase (PPL) was immobilized onto the graphene oxide-based magnetic nanoparticles via covalent bonding. The results show that the novel supporting material CPS/GO-Fe3O4@ MCM-41 was the best for PPL immobilization compared to two other nanomaterials (GO-Fe3O4@ MCM-41 and GO-Fe3O4). The supporting material CPS/GO-Fe3O4@ MCM-41 exhibited enhanced immobilization efficiency (up to 98%), maximum relative activity (up to 97.9%), high stability and reusability (85% after 56 d and 87% after 10 cycles respectively, both at 30 degrees C). Additionally, it offered some other advantages, such as easy recycling and reuse, complying with the requirements of green chemistry. Therefore, it is proposed that CPS/GO-Fe3O4@ MCM-41 provides a new approach for commercial applications.