▎ 摘　　要

A new type of electrochemical aptasensor was reported here for highly sensitive detection of adenosine triphosphate (ATP) and adenosine deaminase (ADA) activity using functionalized graphene as efficient electrochemical label. The specific binding of ATP and its aptamer could link the split aptamers modified graphene and magnetic beads together. After ADA catalysis and magnetic separation, graphene material anchored on electrode surface would efficiently facilitate electron transfer, thus produce detectable electrochemical signals. The detection limits for ATP and ADA activity were 13.6 nM and 0.01 unit/mL (similar to 1.2 nM), respectively. Our work would supply new horizons for the diagnostic applications of graphene-based materials in biomedicine and biosensors. (C) 2014 Elsevier B.V. All rights reserved.