• 文献标题:   Graphene oxide flake activation via divinylsulfone - a procedure for efficient beta-galactosidase immobilization
  • 文献类型:   Article
  • 作  者:   TRUSEK A
  • 作者关键词:   graphene oxide, enzyme chemical immobilization, divinylsulfone, betagalactosidase, lactosefree product
  • 出版物名称:   POLISH JOURNAL OF CHEMICAL TECHNOLOGY
  • ISSN:   1509-8117 EI 1899-4741
  • 通讯作者地址:   Wroclaw Univ Sci Technol
  • 被引频次:   1
  • DOI:   10.2478/pjct-2019-0006
  • 出版年:   2019

▎ 摘  要

Flaky graphene oxide was activated with divinylsulfone followed by immobilization of the beta-galactosidase enzyme. An active and stable preparation was obtained. beta-galactosidase stability after immobilization was much higher than with the native enzyme. The half-life time of the immobilized enzyme was estimated as 165 hours, while for the native form, the estimate was only 5 hours. The developed procedure for the preparation of flaked graphene and its use in the chemical immobilization of enzymes can be used for any enzyme. A processing solution for continuous operation was proposed and verified using cow's milk, with lactose as the hydrolysed substrate, as a dosing stream. Lactose, a milk sugar, was effectively hydrolysed. Product for allergy sufferers who cannot digest lactose has been obtained in this way.