• 文献标题:   Gold Nanoparticles Assembled Chemically Functionalized Reduced Graphene Oxide Supported Electrochemical Immunosensor for Ultra-Sensitive Prostate Cancer Detection
  • 文献类型:   Article
  • 作  者:   BARMAN SC, HOSSAIN MF, PARK JY
  • 作者关键词:  
  • 出版物名称:   JOURNAL OF THE ELECTROCHEMICAL SOCIETY
  • ISSN:   0013-4651 EI 1945-7111
  • 通讯作者地址:   Kwangwoon Univ
  • 被引频次:   8
  • DOI:   10.1149/2.1461706jes
  • 出版年:   2017

▎ 摘  要

An ultra-sensitive electrochemical immunosensor was successfully developed to accurately detect prostate specific antigen. To improve the performance of immunosensor, it was fabricated by using hybrid nano-structured electrodes of high performance chemically functionalized reduced graphene oxide (CRGO) and gold nanoparticles (AuNPs) followed by covalently immobilized monoclonal anti-PSA antibody. We optimized the immunosensor, under different conditions using cyclic voltammetry and differential pulse voltammetry. We obtained a low detection limit of 3 pg mL(-1) and wide linear range from 6 pg mL(-1) to 30 ng mL(-1), good reproducibility, selectivity, and stability. Finally, human serum samples were analyzed. The obtained recovery range was from 97% to 110% with relative standard deviation (RSD) of 3.88%. Thus, the developed immunosensor can provide a great potential in clinical and diagnostic applications. (C) 2017 The Electrochemical Society. All rights reserved.